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KMID : 0613820020120050555
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Journal of Life Science
2002 Volume.12 No. 5 p.555 ~ p.560
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An efficient and reliable electroelution method from SDS-PAGE: Identification of a 31 kDa protein in the postsynaptic density fraction as adenine nucleotide translocator 1
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Jung Jae-Seob
Cho Sun-Jung
Shin Seung-Chul
Jin Ingn-Yol
Jung Yong-Wook
Ko Bok-Hyun
Moon Il-Soo
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Abstract
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The molecular composition of the postsynaptic density (PSD) is largely unknown. In this report, an electroelution protocol was demonstrated to be used for efficient isolation of PSD proteins with diverse molecular sizes. Using this protocol, a 31 kDa protein in the 1% n-octyl glucoside-insoluble PSD fraction (termed as PSD31) was purified from SDS-gels, and internal peptides were determined for amino acid sequences. The amino acid sequences of the PSD31 were highly homologous with the adenine nucleotide translocator 1 (ANT1). The association of ANT1 with PSD suggests presence of a mechanism in synapses for releasing adenosine nucleotides into the extracellular space.
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KEYWORD
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ANT1, electroelution, PSD, protein sequencing, SDS-PAGE
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